Latest Publication:

Purinergic signalling selectively modulates maintenance but not repair neurogenesis in the zebrafish olfactory epithelium.

Demirler MC, Sakizli U, Bali B, Kocagöz Y, Eski SE, Ergönen A, Alkiraz AŞ, Bayramli X, Hassenklöver T, Manzini I, Fuss SH
FEBS Journal 2019,
Olfactory sensory neurons (OSNs) of the vertebrate olfactory epithelium (OE) undergo continuous turnover but also regenerate efficiently when the OE is acutely damaged by traumatic injury. Two distinct pools of neuronal stem/progenitor cells, the globose (GBCs), and horizontal basal cells (HBCs) have been shown to selectively contribute to intrinsic OSN turnover and damage‐induced OE regeneration, respectively. For both types of progenitors, their rate of cell divisions and OSN production must match the actual loss of cells to maintain or to re‐establish sensory function. However, signals that communicate between neurons or glia cells of the OE and resident neurogenic progenitors remain largely elusive. Here, we investigate the effect of purinergic signaling on cell proliferation and OSN neurogenesis in the zebrafish OE. Purine stimulation elicits transient Ca2+ signals in OSNs and distinct non‐neuronal cell populations, which are located exclusively in the basal OE and stain positive for the neuronal stem cell marker Sox2. The more apical population of Sox2‐positive cells comprises evenly distributed glia‐like sustentacular cells (SCs) and spatially restricted GBC‐like cells, whereas the more basal population expresses the HBC markers keratin 5 and tumor protein 63 and lines the entire sensory OE. Importantly, exogenous purine stimulation promotes P2 receptor‐dependent mitotic activity and OSN generation from sites where GBCs are located but not from HBCs. We hypothesize that purine compounds released from dying OSNs modulate GBC progenitor cell cycling in a dose‐dependent manner that is proportional to the number of dying OSNs and, thereby, ensures a constant pool of sensory neurons over time.

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Research Papers:

  • Demirler MC, Sakizli U, Bali B, Kocagöz Y, Eski SE, Ergönen A, Alkiraz AŞ, Bayramli X, Hassenklöver T, Manzini I, Fuss SH (2019) Purinergic signalling selectively modulates maintenance but not repair neurogenesis in the zebrafish olfactory epithelium.
    FEBS Journal, Dec 10. doi: 10.1111/febs.15170

  • Bayramlı X, Kocagöz Y, Sakızlı U, and Fuss SH (2017) Patterned Arrangements of Olfactory Receptor Gene Expression in Zebrafish are Established by Radial Migration of Specified Olfactory Sensory Neurons.
    Scientific Reports, 7(1):5572-87.

  • Fuss SH, Zhu Y, and Mombaerts P. (2013)
    Odorant receptor gene choice and axonal wiring in mice with deletion mutations in the odorant receptor gene SR1.
    Mol Cell Neurosci. 56:212-224.
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  • Kuser-Abali G, Ozcan F, Ugurlu A, Uysal A, Fuss SH, and Bugra-Bilge K. (2013)
    SIK2 Is Involved in the Negative Modulation of Insulin-Dependent Muller Cell Survival and implicated in Hyperglycemia Induced Cell Death.
    Invest. Ophthalmol. Vis. Sci., doi: 10.1167/iovs.12-10729.
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  • Grosmaitre X, Fuss SH, Lee AC, Adipietro KA, Matsunami H, Mombaerts P, and Ma M (2009)
    SR1, a Mouse Odorant Receptor with an Unusually Broad Response Profile
    J. Neurosci., 29: 14545-14552.
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  • Bozza T, Vassalli A, Fuss S, Zhang JJ, Weiland B, Pacifico R, Feinstein, P, and Mombaerts P (2009)
    Mapping of Class I and Class II Odorant Receptors to Glomerular Domains by Two Distinct Types of Olfactory Sensory Neurons in the Mouse.
    Neuron, 61(2):220-233.
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  • Fuss SH, Omura M, and Mombaerts P (2007)
    Local and cis effects of the H element on expression of odorant receptor genes in mouse.
    Cell, 130:373-384.
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  • Fuss SH, Omura M, and Mombaerts P (2005)
    The Grueneberg ganglion of the mouse projects axons to glomeruli in the olfactory bulb.
    Eur J Neurosci, 22:2649-2654.
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  • Çelik A, Fuss SH, Korsching SI (2002)
    Selective targeting of zebrafish olfactory receptor neurons by the endogenous OMP promoter.
    Eur J Neurosci, 15:798-806.
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  • Fried HU, Fuss SH, Korsching SI (2002)
    Selective imaging of presynaptic activity in the mouse olfactory bulb shows concentration and structure dependence of odor responses in identified glomeruli.
    Proc Natl Acad Sci U S A, 99:3222-3227.
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  • Fuss SH, Korsching SI (2001)
    Odorant feature detection: activity mapping of structure response relationships in the zebrafish olfactory bulb.
    J Neurosci, 21:8396-8407.
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Reviews / Previews:

  • Genc S, Zadeoglulari Z, Fuss SH, Genc K. (2012)
    The adverse effects of air pollution on the nervous system.
    J Toxicol.2012:782462.

  • Bayramli X and Fuss SH. (2012)
    Born to run: patterning the Drosophila olfactory system.
    Developmental Cell, 22:240-241.
    Preview for Song et al., Developmental Cell, 22:363-376


  • Fuss SH. and Ray A. (2009)
    Mechanisms of odorant receptor gene choice in Drosophila and vertebrates.
    Molecular and Cellular Neuroscience , 41:101-112.
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  • Fuss S, Celik A, Desplan C (2007)
    Olfactory identity kicked up a NOTCH.
    Nat Neurosci, 10:138-140
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Personal Theses:

  • Fuss S (2002)
    Die Ebenen der neuronalen Informationsverarbeitung im Bulbus olfactorius des Zebrabärblings (Danio rerio): Aktivitätskartierung strukturabhängiger Repräsentation von Geruchsstoffen und Identifizierung molekulargenetischer Marker der einzelnen Verarbeitungsebenen.
    Ph.D. thesis, Institut für Genetik, Universität zu Köln, Germany
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  • Fuss S (1997)
    Identifizierung und Charakterisierung des serotonergen Neurons CV1 der Weinbergschnecke, Helix pomatia.
    Masters thesis, Institut für Zoologie, Johannes-Gutenberg-Universität, Mainz, Germany


Meeting Contributions / Published Abstracts:

  • Xavier Grosmaitre, Stefan Fuss, Peter Mombaerts, Minghong Ma, The septal organ expresses broadly tuned olfactory receptors AChemS / ISOT, 2008, p427

  • Kadioğlu O., Tinaztepe, E., Bayramli, X. and Fuss S.H., Molecular genetic and transgenic approaches to zebrafish olfaction. EMBO Young Investigator Forum, Istanbul 20-22/02/2008

  • Stefan H. Fuss, Masayo Omura and Peter Mombaerts, Regulation of odorant receptor gene expression by the H element in mouse. Jahrestagung der Deutschen Zoologischen Gesellschaft 2007; XXX:XXX

  • Stefan H. Fuss, Arzu Celik and Sigrun I. Korsching, Levels of odorant information processing in the zebrafish olfactory bulb.
    Proceedings of the 4th Meeting of the German Neuroscience Society 2001; Volume II, 28th Göttingen Neurobiology Conference, edited by Norbert Elsner and Georg W. Kreutzberg, Thieme Verlag (2001): 474.

  • S.H. Fuss, A. Celik, S.I. Korsching, Cell type-specific genetic markers in the zebrafish olfactory system.
    Europ J Neurosci, Volume 12, Supplement 11 (2000): 223.07.

  • Stefan H. Fuss and Sigrun I. Korsching, Activity mapping of structure response relationships in the zebrafish olfactory bulb.
    Proceedings of the 1st Göttingen Conference of the German Neuroscience Society 1999, Volume II, 27th Göttingen Neurobiology Conference, edited by Norbert Elsner and Ulf Eysel, Thieme Verlag (1999): 374.

  • Korsching, S.I., Argo, S., Berger, S., Celik, A. and Fuss, S., How are spatial features of olfactory receptor neuron wiring and odorant receptor gene expression established during development?
    Proceedings of the 1st Göttingen Conference of the German Neuroscience Society 1999, Volume II, 27th Göttingen Neurobiology Conference, edited by Norbert Elsner and Ulf Eysel, Thieme Verlag (1999).

  • Sigrun I. Korsching, Rainer W. Friedrich, Stefan Fuss, Franco Weth, Spatial representation of physiological and not so physiological odors in the zebrafish olfactory bulb.
    XIII ECRO congress, Siena, Italy, 1998

  • S. Fuss, L. Ozolina, M. Peschel and T. Teyke, Serotonergic modulation of the tentacle musculature in Helix - cellular origin, biochemical mechanisms and functional aspects.
    Verhandlungen der Deutschen Zoologischen Gesellschaft, Mainz, 1997

  • Stefan Fuss and Thomas Teyke, Serotonergic neuron CV1 is implicated in modulation of tentacle muscle contractions in the snail, Helix pomatia.
    Proceedings of the 24th Göttingen Neurobiology Conference 1996, Volume II, edited by Norbert Elsner and Hans-Ulrich Schnitzler, Thieme Verlag (1996)

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