Latest Publication:

Generation and characterization of inner photoreceptor-specific enhancer-trap lines using a novel piggyBac-Gal4 element in Drosophila.

Terzioğlu-Kara E., Kıral F.R., Öztürk Çolak A., and Çelik A

Archives of Insect Biochemistry and Physiology 2020; 140(2):e21675

The Drosophila inner photoreceptors R7 and R8 are responsible for color vision and their differentiation starts at the third instar larval stage. Only a handful of genes with R7 or R8‐cell‐specific expression are known. We performed an enhancer‐trap screen using a novel piggyBac transposable element, pBGay, carrying a Gal4 sequence under the control of the P promoter to identify novel genes expressed specifically in R7 or R8 cells. From this screen, three lines were analyzed in detail: piggyBacAC109 and piggyBacAC783 are expressed in R8 cells and piggyBacAC887 is expressed in R7 cells at the third instar larval stage and pupal stages. Molecular analysis showed that the piggyBac elements were inserted into the first intron of CG14160 and CG7985 genes and the second intron of unzipped. We show the expression pattern in the developing eye imaginal disc, pupal retina as well as the adult retina. The photoreceptor‐specific expression of these genes is reported for the first time and we propose that these lines are useful tools for studying the development of the visual system.

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Fly lab - Publications


Selected Publications:

  • Bekpen C, Baker C, Hebert MD, Şahin HB, Johnson ME, Çelik A, Mullikin J, NISC Sequencing Program, and Eichler E, Functional characterization of the Morpheus gene family, bioRxiv preprint posted March 12, 2017; doi: http://dx.doi.org/10.1101/116087
  • Çevrim BÇ, Mika K, Özturan G, Kahraman A, Vulstreke V, Callaerts P, and Çelik A (2020) The cell adhesion molecule Unzipped mediates neuron-neuron and neuron-glia interactions to control mushroom body development. in revision Scientific Reports
  • Khoshbakht S, Beheshtian M, Fattahi Z, Bazazzadegan Z, Yilmazer Y, Ataei R, Parsimehr E, Fadaee M, Vazehan R, Zonooz MF, Abolhassani A, Makvand M, Haddadi M, Kariminejad A, Çelik, A, Kahrizi, K., and H. Najmabadi (2020) CEP104 and CEP290; genes with ciliary functions cause intellectual disability in multiple Iranian families. submitted Molecular Genetics and Genomic Medicine
  • Şahin HB, Sayın S, Holder M, Buğra K, and Çelik A (2020) Salt Inducible Kinases as Novel Notch Interactors in the Developing Drosophila Retina. PLoS One, 15(6): e0234744, doi: 10.1371/journal.pone.0234744
  • Terzioğlu Kara E, Kıral FR, Öztürk Çolak A, and Çelik A (2020) Generation and characterization of inner photoreceptor‐specific enhancer‐trap lines using a novel piggyBac‐Gal4 element in Drosophila. Archives of Insect Biochemistry and Physiology 104(2):e21675
  • Dereli Eke E, Arga KY, Dikicioglu D, Eraslan S, Erkol E, Çelik A, Kirdar B, and Di Camillo B (2019) Identification of Novel Components of Target-of-Rapamycin Signaling Pathway by Network-Based Multi-Omics Integrative Analysis OMICS: A Journal of Integrative Biology 23(5):274-284. https://doi.org/10.1089/omi.2019.0021
  • Kazeminasab S, Taşkıran II, Fattahi Z, Bazzazzadegan N, Hosseini M, Rahimi M, Olaad Nabi M, Haddadi M, Çelik A, Ropers HH, Najmabadi H, and K Kahrizi K (2018) CNKSR1 gene defect can cause syndromic autosomal recessive intellectual disability. American Journal of Medical Genetics Part B: Neuropsychiatric Genetics 177(8):691-699. doi: 10.1002/ajmg.b.32648
  • Fattahi Z, Sheikh T, Musante L, Rasheed M, Taşkıran II, Harripaul R, Hu H, Kazeminasab S, Alam MR, Hosseini M, Larti F, Ghaderi Z, Çelik A, Ayub M, Ansar M, Haddadi M, Wienker TF, Ropers HH, Kahrizi K, Vincent JB, and H Najmabadi (2018) Biallelic missense variants in ZBTB11 can cause intellectual disability in human. Human Molecular Genetics 27(18):3177-3188. doi: 10.1093/hmg/ddy220.
  • Köstler S, Alaybeyoğlu B, Weichenberger CX, and Çelik A (2015) FlyOde – a platform for community curation and interactive visualization of dynamic gene regulatory networks in Drosophila eye development. F1000Research 4:1484-9.
  • Potier D, Davie K, Hulselmans G, Sanchez MN, Haagen L, Huynh-Thu VA, Koldere D, Çelik A, Geurts P, Christiaens V, and Aerts S (2014) Mapping gene regulatory networks in Drosophila eye development by large-scale transcriptome perturbations and motif inference. Cell Reports 9:1-14.
  • Mishra AK, Tsachaki M, Rister J, Shy D, Ng J, Çelik A, and Sprecher SG (2013) Binary cell fate decisions and fate transformation in the Drosophila larval eyeila. PLoS Genetics 9(12):e1004027.
  • Li X, Erclik T, Chen Z, Venkatesh S, Morante J, Çelik A, and Desplan C, (2013) Temporal specification of neuroblasts controls neuronal diversity in the Drosophila medulla. Nature 498(7455):456-62.
  • Vasiliauskas D, Mazzoni EO, Sprecher SG, Johnston RJ Jr, Lidder P, Vogt N, Çelik A, and Desplan C (2011) Feedback from Rhodopsin controls Rhodopsin exclusion in Drosophila R8 photoreceptors. Nature 479(7371):108-12.
  • Mazzoni EO, Çelik A, Wernet MF, Vasiliauskas D, Cook TA, Johnston RJ, Pichaud F, and Desplan C (2008) Iroquois-Complex genes induce co-expression of visual pigments in Drosophila. PLoS Biology 6(4):e9.
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    Primer:
    Stavenga DG, and Arikawa K (2008) One Rhodopsin per Photoreceptor: Iro-C Genes Break the Rule. PLoS Biology 6(4):e115.
  • Wernet MF, Çelik A, Mikeladze-Dvali T, and Desplan C (2007) Generation of uniform fly retinas. Current Biology 17(23):r1002-3.
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  • Wernet MF*, Mazzoni EO*, Çelik A*, Duncan DM, Duncan I, and Desplan C (2006) Stochastic spineless expression creates the retinal mosaic for colour vision. Nature 440(7081):174-180. *equal contribution
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    Featured in
    Faculty1000 ("9") and
    Hummel T, and Klaembt C (2006) Eye development: random precision in color vision. Current Biology 16(10):R361-R363.




REVIEWS

  • Şahin HB, and Çelik A (2013) Drosophila eye development and photoreceptor specification eLS, John Wiley & Sons, Ltd: Chichester. DOI:10.1002/9780470015902.a0001147.pub2.
  • Morante J, Desplan C, and Çelik A (2007) Generating patterned arrays of photoreceptors. Current Opinion in Developmental Genetics 17(4):314-9. Review
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  • Fuss S, Çelik A, and Desplan C (2007) Olfactory identity kicked up a Notch. Nature Neuroscience 10(2):138-140. News&Views
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  • Mazzoni EO, Desplan C, and Çelik A (2004) 'One receptor' rules in sensory neurons. Developmental Neuroscience 26(5-6):388-95. Review
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BOOK CONTRIBUTIONS

  • A. Çelik ve Wernet, M.F.W., Editors, "Decoding Neural Circuit Structure and Function: Cellular Dissection Using Genetic Model Organisms," Springer Verlag GmbH DOI 10.1007/978-3-319-57363-2
 ISBN 978-3-319-57362-5
 ISBN 978-3-319-57363-2 (eBook)
 15.07.2017.
  • Scott F. Gilbert, "Gelişim Biyolojisi, Bölüm 7, Amfibiler ve Balıklar: Erken gelişim ve eksen oluşumu," Çeviri: Arzu Çelik, 2013, Nobel Kitapevi.

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